Enzymatic Activity in the M Band

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Enzymatic Activity in the M Band

Experiments which combined histochemistry and electron microscopy were performed in studying the sites of enzymatic hydrolysis of thiolacetic acid in the presence of lead ions in diaphragmatic and cardiac muscle. I t was found that in these striated muscles the electron opaque, final product of the histochemical reaction (PbS) was discretely deposited on the swelling of the thick elemental fila...

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Enzymatic Activity in the M Band

Experiments which combined histochemistry and electron microscopy were performed in studying the sites of enzymatic hydrolysis of thiolacetic acid in the presence of lead ions in diaphragmatic and cardiac muscle. It was found that in these striated muscles the electron opaque, final product of the histochemical reaction (PbS) was discretely deposited on the swelling of the thick elemental filam...

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The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 micro. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescen...

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comparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.

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ژورنال

عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology

سال: 1959

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.6.2.163